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Chronic lactose intake modifies the gastric emptying of monosaccharides but not of disaccharides in weanling rats BJMBR
Ninety-six weanling male Wistar rats were fed for four weeks one of two different chows: a normal rat chow containing 55.5% (w/w) starch (control group, N = 48) or a rat chow in which starch was partially replaced by lactose, in such a way that the experimental group (N = 48) received 35.5% (w/w) starch and 20% (w/w) lactose. The gastric emptying of fluid was then studied by measuring the gastric retention of four test meals containing lactose (5% or 10%, w/v) or glucose + galactose (5% or 10%, w/v). Homogenates of the small intestine were assayed for lactase activity. The gastric retention values were obtained 15 min after orogastric infusion of the liquid meals. The median values for gastric retention of the 5% lactose solutions were 37.7% for the...
Palavras-chave: Gastric emptying; Intestinal motility; Lactase; Lactose.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600004
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Hydrolysis of whey lactose by immobilized β-Galactosidase BABT
Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso.
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
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O leite bovino que produzimos e consumimos. Infoteca-e
PEIXOTO, M. G. C. D.; CARVALHO, M. R. S.; MAGALHAES, V. M. A. de.
Esta cartilha foi elaborada não apenas para desconstruir falsos mitos sobre o leite e derivados, mas também para levar conhecimento ao produtor rural, para que possa tirar dúvidas e prestar esclarecimentos, bem como ao consumidor, para que possa se informar sobre a importância de se incluir os produtos lácteos em sua dieta.
Tipo: Folhetos Palavras-chave: Lactase; ODS 2; ODS 3; ODS 12; Produção Leiteira; Consumo Alimentar; Proteína.
Ano: 2022 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1145076
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Relation of the disaccharidases in the small intestine of the rat to the degree of experimentally induced iron-deficiency anemia BJMBR
Vieira,M.R.; Galvão,L.C.; Fernandes,M.I.M..
Hypolactasia associated with severe iron-deficiency anemia has been reported in several studies. The objective of the present study was to determine whether hypolactasia is associated with the degree and duration of iron-deficiency anemia. Newly weaned male Wistar rats were divided into a control group receiving a diet supplemented with iron (C) and an experimental group (E) receiving a diet not supplemented with iron (iron-deficiency diet). The animals were studied on the 3rd, 5th, 7th, 14th, 21st, 28th and 35th days of the experiment, when overall and iron nutritional status and disaccharidase activity in the small intestine were determined by the Dahlqvist method. A reduction in weight occurred in the anemic animals starting on the 5th day of the study....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disaccharidases; Lactase; Anemia; Iron deficiency; Intestinal mucosa.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500008
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β-galactosidase Encapsulated in Carrageenan, Pectin and Carrageenan/Pectin: Comparative Study, Stability and Controlled Release Anais da ABC (AABC)
SILVA,RENATA CRISTINA; TREVISAN,MARCELLO G.; GARCIA,JERUSA SIMONE.
Abstract The present study investigated the encapsulation of β-galactosidase in carrageenan, pectin and its hybrid hydrogels by using the ionotropic gelation method. The material obtained was characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG/DTG) and scanning electron microscopy (SEM). The effects of pH, temperature and storage time were evaluated in terms of the catalytic activity of the free and encapsulated enzyme. Addition studies were conducted evaluating the performance of catalytic activity in vitro conditions. Carrageenan, pectin and hybrid hydrogels presented encapsulation efficiency of 58 ± 1%, 72 ± 1% and 77 ± 2%, respectively. The pectin hydrogel showed the higher β-galactosidase activity in pH and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopolymer; Encapsulation; Enzymatic activity; Ionotropic gelation; Lactase.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100905
Registros recuperados: 5
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